Quality Assessment of Licorice Based on Quantitative Analysis of Multicomponents by Single Marker Combined with HPLC Fingerprint

Licorice is a commonly used traditional Chinese medicine and natural sweetening agent, rich in numerous bioactive compounds. Moreover, it one of the oldest most frequently employed folk medicines both eastern western countries. It prescribed for treatment asthma, fever, cough. However, with increasing demand licorice, its quality safety become important issue. The content licorice varies significantly materials from different geographical origins. In this study, reasonable feasible evaluation method assessment was developed based on analysis high-performance liquid chromatography (HPLC) fingerprint, combined quantitative multicomponents by single marker (QAMS) method. Glycyrrhizic acid selected as internal reference substance, ten components were simultaneously determined relative correction factors. contents eleven 21 batches QAMS ESM (external standard method); there no significant difference comparison results between method; cosine value (Cir > 0.9999) confirmed consistency two methods. According to outcomes samples, further chemometric analysis. All samples various origins divided into five categories hierarchical cluster analysis, which indicated crucial influence licorice. This study showed that HPLC fingerprint chemometrics methods could effectively control Hence, promising promoting standardization process herbal medicines.